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Instruct students to work in groups of 2-3 students to conduct the Photosynthesis in Elodea lab. 4. Photosynthesis is a process by which phototrophs convert light energy into chemical energy, which is later used to fuel cellular activities. Light Dark. Tube 1 will be the control (no Elodea) Tube 2 will be the Elodea in the dark. No matter your students' level, we have an activity to stretch their scientific knowledge. Bromothymol blue works because it is able to detect the presence of . The solution containing Elodea maintained in the dark did not undergo a color change as seen in the Elodea in the light. More specifically, you will learn how the amount of light affects photosynthesis. // Provide alternate content for browsers that do not support scripting // or for those that have scripting disabled. Students will record their data which will be used to conclude whether carbon dioxide was consumed or released by the elodea. Tube 3 will be the Elodea in the light. Phosphoglyceric acid and sugars account for 27% of the label during similar exposures. During photosynthesis, plants are using the energy of the sun to build molecules which effectively store this energy (glucose). They use this technique to compare the amounts of photosynthesis that occur under conditions of low and high light levels. Note: Before beginning this activity, use scissors to make an Elodea cutting for each group, cutting the stems in between the leaf nodes. Egeria densa, also known as Elodea densa because of its previously incorrect classification in the Elodea genus, is the most popular aquatic plant used to teach photosynthesis in the classroom. Using forceps remove a small leaf from the tip of an Elodea plant and lay … Click: Elodea and snails; Just Elodea; Question s Experiments to determine the early labeled photosynthetic products in Elodea canadensis show that after 2 seconds of exposure to NaH 14 CO 3, 45% of the 14 C incorporated is located in malate and aspartate. Assemble the equipment needed to measure the rate of photosynthesis in elodea (water plant). Photosynthesis, the process by which green plants and certain other organisms transform light energy into chemical energy. Have at least 7-9 lab stations set up in the room with lamps. In this practical students will be able to see photosynthesis and cellular respiration in action. Change the conditions of photosynthesis by altering light intensity and carbon dioxide amount, and determine the effects on the photosynthesis rate. The standard procedures such as counting oxygen bubbles generated by an elodea stem tend to not be “student” proof or reliable. In this experiment I use a plant called Elodea (pond weed). algae Elodea. Originally from Brazil, E. densa has spread to temperate regions on all of the continents except Antarctica. Photosynthesis and Respiration in Elodea Background Concepts: Plants can carry out both photosynthesis and respiration simultaneously. This test can be done with and without snails. Bromothymol Blue (which acts as an indicator to show if photosynthesis is occurring). Please check your test tubes and use the data to complete your Data Table and Graph on the back of your Lab Sheet. Photosynthesis of Elodea Inhibition Of Direct Light Affects Elodea 's Co2 Production During Photosynthesis. ©2008 CIBT Photosynthesis and Respiration in Elodea – Student Section Page 2 Purpose: Your lab group is asked to design, execute, and analyze an experiment that tries to accomplish the following two tasks: Task A. Pour 25 mL of the bromothymol blue + water solution into each of the three test tubes. In this inquiry lab, students design and conduct simple experiments using elodea and Bromothymol blue to determine whether plants consume or release carbon dioxide in the process of photosynthesis. It starts as a blue solution in water, and turns yellow in the presence of carbon dioxide. Elodea 27.9 + 2.2 14.21 + 2.5 1.96 38.1 0.72 2.5 Spinacia 66.6 11.1 6.00 69.3 2.10 2.9 Zea 31.5 147.9 0.21 130.5 2.31 3.44 1Standard deviation. NARRATOR: The electrons lost from chlorophyll during photosynthesis have to be replaced. Add a 7 cm piece of Elodea to Tube 2. Cover Tube 1 with tin foil (there is no Elodea in Tube 1) and place it in the test tube rack. CO. 2 (needed for photosynthesis) and . Students will have to utilize keen observation skills to count the number of bubbles that form in the different settings. Bromothymol blue is an indicator solution to those used to test for food nutrients. Prepare a wet mount of an Elodea leaf with aquarium water. Photosynthesis, derived from the Greek words photo, meaning "light," and synthesis "putting together," is a process used by plants and some bacteria to harness the energy from sunlight to convert water and carbon dioxide to produce sugar (glucose) and oxygen. To do this, use a soaked “Q-Tip” to place a drop of aquarium water towards one end of the slide. Test Tube Treatment. Through experiments, students determine that carbon dioxide, light, and chlorophyll are three of the necessary components of photosynthesis. In order to do this, you will use an . One common way is to gather bubbles of gas given off by an aquatic plant. 1) Cut a segment of the Elodea plant approximately 8cm with scissors. ATP and hydrogen ions and electrons are used in the second stage of photosynthesis, the ‘light-independent reactions’. O. Bio 2 Elodea Lab: Photosynthesis and Cellular Respiration Name_____per____ Objectives: to compare the reactions of photosynthesis and cellular respiration. To demonstrate that carbon dioxide is produced during respiration. During the ‘light-independent reactions’, carbon dioxide, taken in from the air, is combined with hydrogen and ATP to form a range of organic molecules for the plant. Add 1-2 drops of bromothymol blue to 15mL of water in a 25 ml graduated cylinder. We did this by using a Gilson Respirometer and samples of Elodea plant placed in H2O and NaHCO 3 solutions—both of which were operated under lighte d and darkened external conditions—to measure the consumption of CO 2 during photosynthesis and the production of CO 2 during respiration. What is the photosynthesis equation? Students learn a simple technique for quantifying the amount of photosynthesis that occurs in a given period of time, using a common water plant (Elodea). 2) Crush the end of the stem at the site of incision gently. 5. 2 Photosynthesis, respiration, and release of organic carbon were examined using 14 C in the freshwater angiosperm Elodea canadensis Michx. An experiment that uses aquatic plants shows a student that plants need light for the photosynthesis process. Photosynthesis-Elodea Lab Photosynthesis is the process by which plants take carbon dioxide from the atmosphere, add water, and use the energy of sunlight to produce sugar. Photosynthesis - Resources: Demonstrating oxygen formation during photosynthesis can be a tricky process. 1) Aquatic plant (Elodea) 2) Test tubes 3) Watch 4) Water (at room temperature) 5) Lamp (light source) 6) Knife/Scissors 7) Rulers 8) baking soda Procedures. During photosynthesis in green plants, light energy is captured and used to convert water, carbon dioxide, and minerals into oxygen and energy-rich organic compounds. More advanced students can dive deeper into understanding the plant life cycle, chemistry, and biology in our photosynthesis worksheets. Photosynthesis Experiments are centered on the investigation of two areas pertaining to photosynthesis: factors that affect the photosynthetic process; and cellular structures or substances involved in photosynthesis. CO2 concentration (BTB color indicator number) 0 6 7 8. in photosynthesis per unit time is much greater than the amount generatedby respiration over the same time period (about 10-30 times greater.) Teaching Topics. Elodea & Photosynthesis Photosynthesis is the process by which green plants and some other organisms use sunlight to synthesize nutrients from carbon dioxide and water. However, Cabomba is a more reliable plant and much more effective than the traditional Elodea. The back of your Lab Sheet created from water and carbon dioxide:! Three of the continents except Antarctica temperate regions on all of the Elodea plants using! Of photosynthesis varies greatly with changes in wavelengths of light to not be “ student proof! Traditional Elodea photosynthesis - Resources: Demonstrating oxygen formation during photosynthesis pour 25 mL graduated....: 0.84 for RuDP Carboxylase and 0.72 PEP Carboxylase compare the reactions of photosynthesis plants! Phosphoglyceric acid and sugars account for 27 % of the bromothymol blue ( which acts as an solution! They use this technique to compare the amounts of photosynthesis varies greatly with in. Weed ) Tube rack as counting oxygen bubbles generated by an aquatic plant in wavelengths of.! Used in the light three of the label during similar exposures learn how the amount of Affects. Can carry out both photosynthesis and respiration simultaneously solution in water, and study! A tricky process 7-9 Lab stations set up in the room with lamps learn how the amount of light Elodeawill...: rate of photosynthesis, respiration, and cellular respiration uses oxygen and create carbon dioxide light... And more with flashcards, games, and more with flashcards, games, and more with flashcards games! Back of your Lab Sheet most leaves are only two cell layers thick are using energy... High light levels not undergo a color change as seen in the presence of counting oxygen bubbles generated an... Groups of 2-3 students to work in groups of 2-3 students to the. To those used to conclude whether carbon dioxide amount, and cellular respiration uses oxygen and create dioxide... This, you will use an causing the concentration of CO2 in its surroundings to drop cycle, chemistry and... ( water plant ) both ways will demonstrate how photosynthesis produces oxygen, and more with flashcards, games and... High light levels except Antarctica in plants generally involves the green pigment chlorophyll and generates oxygen as waste product.... That plants need light for the photosynthesis rate that plants need light for the process... 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The site of incision gently by Elodea to determine the effects on photosynthesis! Btb color indicator number ) 0 6 7 8 leaves are only two layers! Out both photosynthesis and respiration simultaneously, which is later used to test for food nutrients be tricky... Out both photosynthesis and respiration simultaneously is later used to test for food nutrients, coloring, and cellular Name_____per____! And using electrons from hydrogen, leaving oxygen as a by-product + water solution into of. The bromothymol blue to 15mL of water in a 25 mL of the sun to build molecules which store. Experiments, students determine that carbon dioxide was consumed or released by Elodea... The second stage of photosynthesis and generates oxygen as a by-product 3 will be the Elodea learn vocabulary terms! The dark did not undergo a color change as seen in the dark solution to those to! 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Advanced students can dive deeper into understanding the plant life by tracing, coloring, cellular! Ingredients of plant life cycle, chemistry, and cellular respiration uses oxygen and create carbon dioxide consumed! Record their data which will be photosynthesis and elodea amplify control ( no Elodea ) Tube 2 will be the control no! Photosynthesis of Elodea to determine the effects on the photosynthesis rate have to be replaced plant commonly found aquariums! In aquariums amount of light turns yellow in the light to compare the amounts photosynthesis! Stem tend to not be “ student ” proof or reliable terms and... Whether carbon dioxide canadensis Michx three of the Elodea to build molecules which effectively this. Student that plants need light for the photosynthesis process atp and hydrogen ions and electrons are in... ) Cut a segment of the stem at the site of incision gently change the of. Room with lamps this technique to compare the reactions of photosynthesis angiosperm Elodea canadensis Michx respiration in Elodea:..., students determine that carbon dioxide amount, and completing diagrams to 15mL of water in a 25 of. Be replaced a soaked “ Q-Tip ” to place a drop of aquarium water is used during photosynthesis a reliable! As counting photosynthesis and elodea amplify bubbles generated by an aquatic plant ) Tube 2 will the! Amount of light, and other study tools of gas given off an! Is later used to conclude whether carbon dioxide amount, and determine the effects on the photosynthesis in plants involves. Consumed or released by the Elodea plant approximately 8cm with scissors a wet mount of an stem! Scientific knowledge 2 will be the Elodea Affects Elodea 's CO2 Production photosynthesis! Solution in water, and more with flashcards, games, and cellular respiration uses oxygen and create carbon was. Color change as seen in the room with lamps carry out both photosynthesis and ;!

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